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Aim To study on the protective effects on vein endothelial cell of scallop skite-glycosaminoglycan(SS-GAG)and the mechanism of anti-atherosclerosis action of SS-GAG.Methods The endothelial cell of human umbilical vein had been cultured in vitro, and we established an model of endothelial cell oxidative damage induced by oxidized low density lipoprotein (OX-LDL), MTT assay and chemical methods were used to test the influence of SS-GAG on proliferation activity of endothelial cell oxidative damage and analyze nitric oxide (NO) and endothelial nitric oxide synthase (eNOS).Results Oxidized lowdensity lipoprotein (OX-LDL) remarkably inhibited the ability of cell proliferation, decreased nitric oxide (NO) and endothelial nitric oxide synthase (eNOS) (P
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Objective To establish a sandwich enzyme linked immunosorbent assay (ELISA) method for determination of von Willebrand factor propeptide (vWF-pp) in plasma and to determine the levels of plasma vWF-pp in 152 healthy volunteers, 36 patients with coronary heart disease and 38 cases of the normal control group with the same ages.Methods The levels of vWF-pp in 152 healthy volunteers with different ages and different genders and in 36 patients with coronary heart disease were determined with the sandwich enzyme linked immuno-sorbent assay (ELISA).Results The average level of vWF-pp in 152 healthy volunteers was 496.4?167.2 ?g/L.The level of vWF-pp in the 50~60 years old group was significantly higher than that in the less than 40 years old group (P0.05). There was significant difference between the 51～60 years old male group and the other three male groups respectively, the 16～20 years old group, the 21～30 years old group and the 31～40 years old group (P
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AIM:To investigate effects of propolis-ethanol extract on platelet activity in flowing blood.METHODS: Intima-injury model was made by in vitro perfusion.Coverslips was coated by using fibrinogen and human Ⅲ type collagen to detect platelet adhesive rate when blood went through fibrinogen and collagen surface in 1 000/s shear rate.Three experimental groups were set up: negative control group(24% ethanol),experimental group(0.1 g/L,24% propolis-ethanol extract) and positive control group(0.1 g/L,ferulic acid).RESULTS: Intima-injury model was made.On fibrinogen and collagen,platelet adhesion on area of experimental group was lower than that in negative control group(P0.05).Propolis-ethanol extract decreased more obviously the platelet adhesion area on fibrinogen surface than that in collagen surface(P